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rabbit anti a1r (Proteintech)

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Proteintech rabbit anti a1r
Rabbit Anti A1r, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+a1r/Adenosine+A1+Receptor+Antibody/pm41781500-420-3-7
Average 93 stars, based on 19 article reviews

rabbit anti a1r - by Bioz Stars, 2026-10

93/100 stars

Images

Western Blot:

Article Title: Adenosine deficiency facilitates CA1 synaptic hyperexcitability in the presymptomatic phase of a knock in mouse model of Alzheimer’s disease
Article Snippet: SeeBlue Plus2 standard (#LC5925, Life Technologies) was used to estimate protein sizes, and transfer was confirmed by Ponceau S (#BP103-10, Fisher Biotech) staining. .. Immunoblot was obtained by first blocking membranes for 1 h at room temperature with a solution of 0.1% Tween 20 and 5% Bovine Serum Albumin (BSA) (#5217, Tocris) in 1× PBS and then incubated with primary antibodies overnight at 4°C (rabbit anti-ADK 1:2000, #A304-280A-T, Fortis Life Sciences; mouse anti-SAHH 1:500, #sc-271389, Santa Cruz Biotechnology; rabbit anti-ADA 1:1000, #A5151, AbClonal; rabbit anti-CD39 1:1000, #14481, Cell Signaling; rabbit anti-NT5 1:1000, #13160, Cell Signaling; rabbit anti-A1R 1:1000, #55026-1-AP, Proteintech; rabbit anti-ENT1 1:500, #11337-1-AP, Proteintech). .. After washing with 1× PBS Containing 0.1% Tween 20, the membranes were incubated with the species-appropriate horseradish peroxidase-conjugated secondary antibodies (HRP-conjugated goat polyclonal anti-rabbit #31460, Thermofisher; or HRP-conjugated goat polyclonal anti-mouse #31430, Thermo Scientific) for 1 h at room temperature at a 1:20,000 dilution in blocking solution.

Article Title: Adenosine deficiency facilitates CA1 synaptic hyperexcitability in the presymptomatic phase of a knockin mouse model of Alzheimer disease
Article Snippet: SeeBlue Plus2 standard (#LC5925, Life Technologies) was used to estimate protein sizes, and transfer was confirmed by Ponceau S (#BP103-10, Fisher Biotech) staining. .. Immunoblot was obtained by first blocking membranes for 1 h at room temperature with a solution of 0.1% Tween 20 and 5% Bovine Serum Albumin (BSA) (#5217, Tocris) in 1× PBS and then incubated with primary antibodies overnight at 4°C (rabbit anti-ADK 1:2000, #A304-280A-T, Fortis Life Sciences; mouse anti-SAHH 1:500, #sc-271389, Santa Cruz Biotechnology; rabbit anti-ADA 1:1000, #A5151, AbClonal; rabbit anti-CD39 1:1000, #14481, Cell Signaling; rabbit anti-NT5 1:1000, #13160, Cell Signaling; rabbit anti-A1R 1:1000, #55026-1-AP, Proteintech; rabbit anti-ENT1 1:500, #11337-1-AP, Proteintech). .. After washing with 1× PBS Containing 0.1% Tween 20, the membranes were incubated with the species-appropriate horseradish peroxidase-conjugated secondary antibodies (HRP-conjugated goat polyclonal anti-rabbit #31460, Thermofisher; or HRP-conjugated goat polyclonal anti-mouse #31430, Thermo Scientific) for 1 h at room temperature at a 1:20,000 dilution in blocking solution.

Blocking Assay:

Incubation:

other:

Labeling:

Article Title: 40 Hz light flickering alleviates chronic pain via adenosine signaling in the retina-amygdala pathway.
Article Snippet: For A2AR labeling, goat anti-A2AR (1:500, A2A-Go-Af700, Frontier Institute) was applied for 24 h at 4 °C, followed by donkey anti-goat Alexa Fluor 488 (1:1000, A11055, Invitrogen) for 2 h at room temperature. .. For A1R labeling, rabbit anti-A1R (1:500, 20332-1-AP, Proteintech) was applied for 24 h at 4 °C, followed by goat anti-rabbit Alexa Fluor 568 (1:1000, A11011, Invitrogen) for 2 h at room temperature. .. For CRF labeling, rabbit anti-CRF (1:200, 10944-1-AP, Proteintech) was applied for 24 h at 4 °C, followed by goat anti-rabbit Alexa Fluor 568 (1:1000, A11011, Invitrogen) for 2 h at room temperature.

Image Search Results

The effect of intraperitoneal injection of A1R antagonist on the therapeutic effect of EA. A Experimental scheme of CFA modeling, drug injection, EA intervention and behavioral tests. B DPCPX decreased the effect of EA on PWTs. C – G Results of OF. C Representative exploration traces of OF. D Time in center of OF (F 2,33 = 13.33, P < 0.0001). E Entries in center of OF (F 2,33 = 7.583, P = 0.0020). F Distance in center of OF (F 2,33 = 5.780, P = 0.0070). G Anxiety index of OF (F 2,33 = 4.242, P = 0.0199). H – K Results of EPM. H Representative exploration traces in EPM. I Time in open arm of EPM (F 2,33 = 12.16, P = 0.0001). J Entries in open arm of EPM (F 2,33 = 13.12, P < 0.0001). K Anxiety index of EPM (F 2,33 = 9.437, P = 0.0006). L – M Results of NSF. L Latency to food (F 2,33 = 224.74, P < 0.0001). M Food consumption (F 2,33 = 00.1098, P = 0.88964). N Total distance (F 2,33 = 0.5596, P = 0.5767). O Emotional z score (F 2,33 = 13.36, P < 0.0001). P Representative for the c-Fos and PV positive cells in the contralateral ACC. Q Result of c-Fos positive cell in the contralateral ACC (F 2,9 = 6.537, P = 0.0176). R Result of PV positive cell in the contralateral ACC (F 2,9 = 8.602, P = 0.0082). S Representative for the c-Fos and PV positive cells in the ipsilateral ACC. T Date representative for c-Fos positive cells in the ipsilateral ACC shown similarly to Q (F 2,9 = 6.912, P = 0.0152). U Date representative for PV positive cells in the ipsilateral ACC shown similarly to R (F 2,9 = 17.44, P = 0.0008). * P < 0.05, ** P < 0.01, M-PBS v.s. M-EA-PBS; # P < 0.05, ## P < 0.01, M-EA-PBS v.s. M-EA-DPCPX; ns, no significant different ( P > 0.05). Data are presented as the means ± SEM. n = 11–13 mice for all mouse group. One-way ANOVA plus post hoc Tukey test

Journal: Chinese Medicine

Article Title: Astrocyte inhibition and PV interneuron activation: key mechanisms in electroacupuncture's effect on pain-anxiety comorbidity

doi: 10.1186/s13020-025-01202-1

Figure Lengend Snippet: The effect of intraperitoneal injection of A1R antagonist on the therapeutic effect of EA. A Experimental scheme of CFA modeling, drug injection, EA intervention and behavioral tests. B DPCPX decreased the effect of EA on PWTs. C – G Results of OF. C Representative exploration traces of OF. D Time in center of OF (F 2,33 = 13.33, P < 0.0001). E Entries in center of OF (F 2,33 = 7.583, P = 0.0020). F Distance in center of OF (F 2,33 = 5.780, P = 0.0070). G Anxiety index of OF (F 2,33 = 4.242, P = 0.0199). H – K Results of EPM. H Representative exploration traces in EPM. I Time in open arm of EPM (F 2,33 = 12.16, P = 0.0001). J Entries in open arm of EPM (F 2,33 = 13.12, P < 0.0001). K Anxiety index of EPM (F 2,33 = 9.437, P = 0.0006). L – M Results of NSF. L Latency to food (F 2,33 = 224.74, P < 0.0001). M Food consumption (F 2,33 = 00.1098, P = 0.88964). N Total distance (F 2,33 = 0.5596, P = 0.5767). O Emotional z score (F 2,33 = 13.36, P < 0.0001). P Representative for the c-Fos and PV positive cells in the contralateral ACC. Q Result of c-Fos positive cell in the contralateral ACC (F 2,9 = 6.537, P = 0.0176). R Result of PV positive cell in the contralateral ACC (F 2,9 = 8.602, P = 0.0082). S Representative for the c-Fos and PV positive cells in the ipsilateral ACC. T Date representative for c-Fos positive cells in the ipsilateral ACC shown similarly to Q (F 2,9 = 6.912, P = 0.0152). U Date representative for PV positive cells in the ipsilateral ACC shown similarly to R (F 2,9 = 17.44, P = 0.0008). * P < 0.05, ** P < 0.01, M-PBS v.s. M-EA-PBS; # P < 0.05, ## P < 0.01, M-EA-PBS v.s. M-EA-DPCPX; ns, no significant different ( P > 0.05). Data are presented as the means ± SEM. n = 11–13 mice for all mouse group. One-way ANOVA plus post hoc Tukey test

Article Snippet: The following primary antibodies were used: rabbit anti-c-Fos (1:1000, ab190289, Abcam, USA), rabbit anti-PV (1:500, ab181086, Abcam, USA), rabbit anti-GFAP (1:1000, ab7260, Abcam, USA), rabbit anti-Iba1 (1:1000, ab178864, Abcam, USA), rabbit anti-NeuN (1:1000, ab177487, Abcam, USA), and rabbit anti-adenosine A1R (1:200, NB300549, Novus Biologicals, USA).

Techniques: Injection

Microinjecting the A1R antagonist reversed the effect of inhibition of GFAP. A Experimental scheme of virus injection, CFA injection, drug administration, and behavioral tests. B Virus injection strategy (left) and representative image show location of virus expression (right). C The impact of DPCPX (i.v.) on the effect of GFAP inhibition on PWTs (on 18d, t = 12.24, P < 0.0001). D – I Results of OF. D Representative exploration traces of OF. E Time in center of OF (t = 5.223, P < 0.0001). F Entries in center of OF (t = 6.530, P < 0.0001). G Distance in center of OF (t = 6.023, P < 0.0001). H Total distance of OF (t = 1.044, P = 0.3083). I Anxiety index of OF (t = 6.508, P < 0.0001). J – M Result of EPM. J Representative exploration traces of EPM. K Time in open arm of EPM (t = 3.315, P = 0.0033). L Entries in open arm of EPM (t = 4.807, P < 0.0001). M Anxiety index of EPM (t = 4.079, P = 0.0005). N – O Result of NSF. N Latency to food (t = 3.401, P = 0.00330). O Food consumption (t = 0.77692, P = 0.44512). P Emotional z score (t = 5.902, P < 0.0001). $$ P < 0.01, compared with the pre-CFA injection of M-GFAP-hM4Di-ASCF; ## P < 0.01, compared with the 12d after CFA injection of M-GFAP-hM4Di-ASCF; ** P < 0.01, M-GFAP-hM4Di-ASCF v.s. M-GFAP-hM4Di-DPCPX; ns, no significant different ( P > 0.05). n = 10–11 mice/group. Data are presented as the means ± SEM. Two-tailed unpaired t -test

Journal: Chinese Medicine

Article Title: Astrocyte inhibition and PV interneuron activation: key mechanisms in electroacupuncture's effect on pain-anxiety comorbidity

doi: 10.1186/s13020-025-01202-1

Figure Lengend Snippet: Microinjecting the A1R antagonist reversed the effect of inhibition of GFAP. A Experimental scheme of virus injection, CFA injection, drug administration, and behavioral tests. B Virus injection strategy (left) and representative image show location of virus expression (right). C The impact of DPCPX (i.v.) on the effect of GFAP inhibition on PWTs (on 18d, t = 12.24, P < 0.0001). D – I Results of OF. D Representative exploration traces of OF. E Time in center of OF (t = 5.223, P < 0.0001). F Entries in center of OF (t = 6.530, P < 0.0001). G Distance in center of OF (t = 6.023, P < 0.0001). H Total distance of OF (t = 1.044, P = 0.3083). I Anxiety index of OF (t = 6.508, P < 0.0001). J – M Result of EPM. J Representative exploration traces of EPM. K Time in open arm of EPM (t = 3.315, P = 0.0033). L Entries in open arm of EPM (t = 4.807, P < 0.0001). M Anxiety index of EPM (t = 4.079, P = 0.0005). N – O Result of NSF. N Latency to food (t = 3.401, P = 0.00330). O Food consumption (t = 0.77692, P = 0.44512). P Emotional z score (t = 5.902, P < 0.0001). $$ P < 0.01, compared with the pre-CFA injection of M-GFAP-hM4Di-ASCF; ## P < 0.01, compared with the 12d after CFA injection of M-GFAP-hM4Di-ASCF; ** P < 0.01, M-GFAP-hM4Di-ASCF v.s. M-GFAP-hM4Di-DPCPX; ns, no significant different ( P > 0.05). n = 10–11 mice/group. Data are presented as the means ± SEM. Two-tailed unpaired t -test

Techniques: Inhibition, Virus, Injection, Expressing, Two Tailed Test

( A ) Representative confocal images of dendrites of cultured Syt11 +/+ and Syt11 −/− hippocampal neurons (DIV14). Neurons were incubated with Transferrin-AF647 (magenta) for 30 min to label early endosomes. Fixed and permeabilized neurons were then stained for endogenous GB2, Cav2.2, or A1R (all green). Arrowheads indicate examples of Transferrin-AF647+ vesicles carrying GB2, Cav2.2, or A1R. Scale bar, 5 μm. Increased Transferrin-AF647 uptake is observed in Syt11 −/− compared to Syt11 +/+ neurons. n = 21 neurons for each genotype from 3 independent experiments. ( B ) Quantification of co-localization of GB2, Cav2.2, or A1R with Transferrin-AF647 in experiments described in ( A ). The Pearson’s correlation coefficients indicate the degree of co-localization between Transferrin-AF647 and GB2, Cav2.2, or A1R in dendrites. In Syt11 −/− neurons, co-localization with Transferrin-AF647 is increased for endogenous GB2 and Cav2.2, indicating increased internalization. GB2, n = 27 neurons; Cav2.2, n = 18 neurons; A1R, n = 20 neurons from 3 independent experiments. ( C ) Representative confocal images of dendrites of cultured Syt11 +/+ and Syt11 −/− hippocampal neurons (DIV14) stained for endogenous GB2 (green) and the lysosome marker LAMP1 (magenta). Arrowheads indicate examples of co-localization of GB2 with LAMP1. Scale bar, 5 μm. Pearson’s correlation coefficient indicates increased co-localization of GB2 with LAMP1 in dendrites of Syt11 −/− neurons. Syt11 +/+ , n = 18 neurons; Syt11 −/− , n = 17 neurons from 3 independent experiments. Data information: Data are presented as mean ± SEM. Statistical significance was determined by Welch’s t -test ( A ), unpaired Student’s t -test ( B ), or Mann–Whitney U test ( C ). ns not significant; ** p < 0.01.

Journal: EMBO Reports

Article Title: Synaptotagmin-11 facilitates assembly of a presynaptic signaling complex in post-Golgi cargo vesicles

doi: 10.1038/s44319-024-00147-0

Figure Lengend Snippet: ( A ) Representative confocal images of dendrites of cultured Syt11 +/+ and Syt11 −/− hippocampal neurons (DIV14). Neurons were incubated with Transferrin-AF647 (magenta) for 30 min to label early endosomes. Fixed and permeabilized neurons were then stained for endogenous GB2, Cav2.2, or A1R (all green). Arrowheads indicate examples of Transferrin-AF647+ vesicles carrying GB2, Cav2.2, or A1R. Scale bar, 5 μm. Increased Transferrin-AF647 uptake is observed in Syt11 −/− compared to Syt11 +/+ neurons. n = 21 neurons for each genotype from 3 independent experiments. ( B ) Quantification of co-localization of GB2, Cav2.2, or A1R with Transferrin-AF647 in experiments described in ( A ). The Pearson’s correlation coefficients indicate the degree of co-localization between Transferrin-AF647 and GB2, Cav2.2, or A1R in dendrites. In Syt11 −/− neurons, co-localization with Transferrin-AF647 is increased for endogenous GB2 and Cav2.2, indicating increased internalization. GB2, n = 27 neurons; Cav2.2, n = 18 neurons; A1R, n = 20 neurons from 3 independent experiments. ( C ) Representative confocal images of dendrites of cultured Syt11 +/+ and Syt11 −/− hippocampal neurons (DIV14) stained for endogenous GB2 (green) and the lysosome marker LAMP1 (magenta). Arrowheads indicate examples of co-localization of GB2 with LAMP1. Scale bar, 5 μm. Pearson’s correlation coefficient indicates increased co-localization of GB2 with LAMP1 in dendrites of Syt11 −/− neurons. Syt11 +/+ , n = 18 neurons; Syt11 −/− , n = 17 neurons from 3 independent experiments. Data information: Data are presented as mean ± SEM. Statistical significance was determined by Welch’s t -test ( A ), unpaired Student’s t -test ( B ), or Mann–Whitney U test ( C ). ns not significant; ** p < 0.01.

Article Snippet: Primary antibodies were rabbit anti-synaptophysin (Cat# ab32127, Abcam, 1:1000), mouse anti-PSD95 (Cat# MA1-045, Thermo Scientific, 1:1000), chicken anti-MAP2 (Cat# ab5392, Abcam, 1:3000), sheep anti-NPY (Cat# ab6173, Abcam, 1:500), guinea pig anti-GABA B2 (Cat# 322 205, Synaptic Systems, 1:1000), rabbit anti-Cav2.2 (Cat# ACC-002, Alomone Labs, 1:1000), rabbit anti-A1R (Cat# ab3460, Abcam, 1:1000) and anti-LAMP1 (Cat# ab208943, Abcam, 1:1000).

Techniques: Cell Culture, Incubation, Staining, Marker, MANN-WHITNEY